DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): implications for hybrid detection

Mark R. Jeschke; Patrick J. Tranel; A. Lane Rayburn

doi:10.1614/0043-1745(2003)051[0001:DCAOSP]2.0.CO;2

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1 January 2003 DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): implications for hybrid detection

Mark R. Jeschke, Patrick J. Tranel, A. Lane Rayburn

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Weed Science, 51(1):1-3 (2003). https://doi.org/10.1614/0043-1745(2003)051[0001:DCAOSP]2.0.CO;2

Abstract

Prior research indicated that analysis of nuclear DNA content by flow cytometry could be used to distinguish smooth pigweed × tall waterhemp hybrids when the parent lines are known. Flow cytometry was performed on nuclei isolated from several Illinois populations of smooth pigweed and tall waterhemp. The smooth pigweed and tall waterhemp analyzed had nonoverlapping 2C nuclear DNA content values, with mean values of 1.04 and 1.34 pg, respectively. The consistent difference in DNA content observed between the two species indicates that DNA content analysis can be used to distinguish their hybrid progeny in natural populations.

Nomenclature: Smooth pigweed, Amaranthus hybridus L. AMACH; tall waterhemp, Amaranthus tuberculatus (Moq.) Sauer AMATU.

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Mark R. Jeschke, Patrick J. Tranel, and A. Lane Rayburn "DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): implications for hybrid detection," Weed Science 51(1), 1-3, (1 January 2003). https://doi.org/10.1614/0043-1745(2003)051[0001:DCAOSP]2.0.CO;2

Received: 20 March 2002; Accepted: 7 May 2002; Published: 1 January 2003

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